Team:Caltech/Week 10

From 2010.igem.org

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==Monday 8/16==
==Monday 8/16==
 +
* Group meeting!
 +
* Ran CPCR gels on L31, L34, L43, L58 & L71
 +
** All negative.
==Tuesday 8/17==
==Tuesday 8/17==
 +
* Took our team photo!
 +
* Ran more CPCR of L58 & L81.
 +
** Gel was negative.
 +
* Cultured HSP-LacI-GFP cells in LB + 1M glucose to try to up-regulate LacI repression of GFP production. Incubated cells at 37°C overnight.
==Wednesday 8/18==
==Wednesday 8/18==
 +
* LacI appears fully repressed in L62 cells after overnight incubation at 37°C.
 +
** Will now spin down cells and resuspend in LB + IPTG to see if overnight incubation now activates GFP production, as predicted.
 +
* Picked more CPCR colonies of L81 & L58 (x6) to try to find plastic production cells.
 +
* Transformed L60 & L61. Plated on plates containing 50μL 50mM glucose (dried).
 +
** We hypothesize that the reason these ligations produced no colonies is due to leaky LacI repression, allowing a low level of lysis expression, eventually killing all the cells. Will see if there are cells on these plates tomorrow.
 +
* Submitted our poster deign for printing for Sasha's SURF presentation tomorrow.
==Thursday 8/19==
==Thursday 8/19==

Revision as of 01:32, 19 August 2010


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Monday 8/16

  • Group meeting!
  • Ran CPCR gels on L31, L34, L43, L58 & L71
    • All negative.

Tuesday 8/17

  • Took our team photo!
  • Ran more CPCR of L58 & L81.
    • Gel was negative.
  • Cultured HSP-LacI-GFP cells in LB + 1M glucose to try to up-regulate LacI repression of GFP production. Incubated cells at 37°C overnight.

Wednesday 8/18

  • LacI appears fully repressed in L62 cells after overnight incubation at 37°C.
    • Will now spin down cells and resuspend in LB + IPTG to see if overnight incubation now activates GFP production, as predicted.
  • Picked more CPCR colonies of L81 & L58 (x6) to try to find plastic production cells.
  • Transformed L60 & L61. Plated on plates containing 50μL 50mM glucose (dried).
    • We hypothesize that the reason these ligations produced no colonies is due to leaky LacI repression, allowing a low level of lysis expression, eventually killing all the cells. Will see if there are cells on these plates tomorrow.
  • Submitted our poster deign for printing for Sasha's SURF presentation tomorrow.

Thursday 8/19

Friday 8/20

 
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