Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/15

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E.coli Pattern Formation Project Notebook



August 2010
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2010/08/15

1:DNA extraction with ISOPLANT(NIPPON GENE)

<Member>
Mariko, easily, nito, Hitomi, Taka


<Sample>
・E-coli (having BBa_I732901 plasmid)


<Protocol>
SeeProtocol 5


2:DNA concentration measurement

<Member>
Mariko, easily, nito, Hitomi, Taka


<Materials, Sample>
・TE

・E-coli (having BBa_I732901 plasmid)


<Protocol>

  1. Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
  2. Fall in drops 1 on the measuring machine.


<Result>
Concentration: 16.8
A320: 0.046
A260/A280: 3.414
A260/A230: 0.062