E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/08/28 1.1 1：PCR 1.2 2：Plasmid extraction from E-coli 1.3 3：DNA concentration measurement 1.4 4：Electrophoresis

2010/08/28

1：PCR

＜Member＞
Mariko, Hitomi

＜Sample＞
・E-coli (having BBa_I732901 plasmid)

＜Protocol＞
See Protocol 2

・Tube(higher temperature/lower temperature in annealing)

1. Rfp (67.5℃/66.0)
2. Terminator (66.0/63.0)

Total 4 tubes.

2：Plasmid extraction from E-coli

＜Member＞
Hitomi

＜Sample＞
・transformed E-coli (8/26)

• BBa_I13521
• BBa_B0010
• BBa_B0030
• BBa_J23110
• BBa_E0040

＜Protocol＞
SeeProtocol 6

3：DNA concentration measurement

＜Member＞
Hitomi

＜Sample＞
・PCR products

＜Protocol＞

1. Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
2. Fall in drops 1 on the measuring machine.

＜Result＞

DNA concentration

	concentration	A320	A260/A280	A260/A230
RH	565.0	20.24	1.723	1.175
RL	400.5	10.73	1.697	1.462
TH	356.0	8.47	1.622	1.201
TL	324.5	8.31	1.627	1.195

4：Electrophoresis

＜Member＞
Mariko, Hitomi

＜Sample＞
・PCR products

＜Protocol＞
SeeProtocol 8

＜Result＞