E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/09/11 1.1 1:PCR 1.2 2:Digestion(1) 1.3 3:Electrophoresis 1.4 4:Electrophoresis 1.5 5:DNA extraction 1.6 6:Digestion(2) 1.7 7:Electrophoresis 1.8 8:DNA extraction 1.9 9:Ligation 1.10 10:DNA extraction 1.11 12:Ligation

2010/09/11

1:PCR

<Member>
mio,easily

<Sample>

• 2

<Protocol>
See Protocol 2

(temperature in annealing)

• 2:70.0℃

2:Digestion(1)

<Member>
Mariko,mizuki

<Sample>

• 1H
• 2(9/6)
• 4(8/30)
• 5(8/30)

<Protocol>
See Prptpcpl 9

3:Electrophoresis

<Member>
Mariko,mio

<Sample>

• PCR products(9/11)
• Digestion products(9/11)

<Protocol>
See Protocol 8

<Result>

4:Electrophoresis

<Member>
mio

<Sample>
Digestion products (9/11)

• 1
• 2
• 4
• 5

<Protocol>
See Protocol 8

<Result>

5:DNA extraction

<Member>
mizuki,easily

<Sample>
PCR products(9/11)

<Protocol>
See Protocol 4

6:Digestion(2)

<Member>
easily

<Sample>

• 1(9/11)

<Protocol>
See Prptpcpl 9

7:Electrophoresis

<Member>
Mariko,mizuki

<Sample>
Digestion products

• 1(9/11)
• 4L(8/26)
• 8H

<Protocol>
See Protocol 8

<Result>

8:DNA extraction

<Member>
mio,mizuki

<Sample>

• 4(9/11)
• 5(9/11)

<Protocol>
See Protocol 4

9:Ligation

<Member>
Mariko,mio

<Sample>

• 4(9/11)
• 5(9/11)

<Protocol>
See Protocol 10

10:DNA extraction

<Member>
Mariko,mio,mizuki

<Sample>

• 1(9/11)
• 2(9/11)

<Protocol>
See Protocol 4

12:Ligation

<Member>
Mariko

<Sample>

• 1(9/11)
• 2(9/11)

<Protocol>
See Protocol 10