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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/15
From 2010.igem.org
E.coli Pattern Formation Project Notebook
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2010/08/15
1：DNA extraction with ISOPLANT(NIPPON GENE)
＜Member＞
Mariko, easily, nito, Hitomi, Taka
＜Sample＞
・E-coli (having BBa_I732901 plasmid)
＜Protocol＞
SeeProtocol 5
2：DNA concentration measurement
＜Member＞
Mariko, easily, nito, Hitomi, Taka
＜Materials, Sample＞
・TE
・E-coli (having BBa_I732901 plasmid)
＜Protocol＞
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
＜Result＞
Concentration: 16.8
A320: 0.046
A260/A280: 3.414
A260/A230: 0.062
