E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/10/07 1.1 1:PCR 1.2 2：DNA extraction 1.3 3:Plasmid extraction from E-coli 1.4 4:Electrophoresis 1.5 5：DNA extraction 1.6 6：DNA extraction from gels

2010/10/07

1:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 4-5
• 2-3
• 2-7
• 6-7
• 2-10

<Protocol>
See Protocol 1

2：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products (10/6)

＜Protocol＞
See Protocol 4

3:Plasmid extraction from E-coli

<Member>
nito,Taka

<Sample>

<Protocol>
See Protocol 6

4:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/7)

<Protocol>
See Protocol 8

<Result>

5：DNA extraction

＜Member＞
mizuki,mio

＜Sample＞
PCR products

• 1
• 4
• 5
• 9
• 10
• 1-2
• 2-10

＜Protocol＞
See Protocol 4

6：DNA extraction from gels

＜Member＞
nito

＜Sample＞
Digestion products

• 9
• 1-2
• 2-10

(After electrophoresis)

＜Protocol＞
See Protocol 4