E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/09/28 1.1 1：DNA extraction 1.2 2：DNA Ligation 1.3 3:PCR 1.4 4:Transformation 1.5 5:Electrophoresis 1.6 6：DNA extraction 1.7 7：DNA Digestion 1.8 8:PCR

2010/09/28

1：DNA extraction

＜Member＞
mizuki,Takahiro

＜Sample＞
PCR products

• 1(9/16)
• 2(9/16)
• 3(9/17)
• 4(9/16)
• 5(9/16)
• 7(9/16)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mio

＜Sample＞

• 7-8-10,vector
• 2,vector

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

• 1 (72)
• 2 (70)
• 4 (70)
• 5 (70)
• 9 (63,68)
• 10(58)
• vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

<Sample>

• 7-8-10+vector
• 2+vector

<Protocol>
See Protocol 7

5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

• 1 (9/28)
• 2 (9/28)
• 4 (9/28)
• 5 (9/28)
• 8 (9/27)
• 9 (9/28)
• 10(9/28)
• vector

<Protocol>
See Protocol 8

<Result>

6：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

• 1 (8/31)
• 2 (9/11)
• 4L(8/26)
• 5 (8/31)

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
Mariko

<Sample>(temperature in annealing:℃)

• 1 (72)
• 2 (70)
• 3 (69)
• 4 (70)

<Protocol>
See Protocol 1