Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/21

E.coli Fiber Project Notebook

2010/10/21 Thursday (Naoto)

member naoto and watachin

Experiment:PCR

material

• templete DNA (from A.xylinum and pSB1C3)

you can see other materials at protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

• PCR production(bcsA and bcsB)

you can see materials at protocol4

procedure

refer to protocol4

Experiment:DNA purification with silica gel

material

• PCR production(bcsD and pSB1C3)

you can see materials at protocol5-2

procedure

refer to protocol5-2

Experiment:Electrophoresis

material

• PCR production (bcsA,bcsB,bcsC and bcsD)

you can see other materials at protocol4

procedure

see protocol4

Experiment:Digestion

material

• bcsC
• bcsD
• XbaI
• SpeI

you can see other materials at protocol6

procedure see protocol6

Experiment:DNA purification with silica gel

material

• PCR production(bcsC and bcsD)

you can see materials at protocol5-2

procedure

refer to protocol5-2

Experiment:Ligation

material

• bcsC and bcsD

you can see materials at protocol7

procedure

refer to protocol7

Experiment:Transformation

material

• Competent cell(Ecos JM109)
• bcsD in pSB1C3

you can see materials at protocol8

procedure see protocol8