Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/21

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E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31


2010/10/21 Thursday (Naoto)

member naoto and watachin

Experiment:PCR

material

  • templete DNA (from A.xylinum and pSB1C3)

you can see other materials at protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production(bcsA and bcsB)

you can see materials at protocol4

procedure

refer to protocol4

Experiment:DNA purification with silica gel

material

  • PCR production(bcsD and pSB1C3)

you can see materials at protocol5-2

procedure

refer to protocol5-2


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/16 Saturday (Naoto)

member naoto and watachin

Experiment:Electrophoresis

material

  • PCR production (bcsA,bcsB,bcsC and bcsD)

you can see other materials at protocol4

procedure

see protocol4

Experiment:Digestion

material

  • bcsC
  • bcsD
  • XbaI
  • SpeI

you can see other materials at protocol6

procedure see protocol6

Experiment:DNA purification with silica gel

material

  • PCR production(bcsC and bcsD)

you can see materials at protocol5-2

procedure

refer to protocol5-2

Experiment:Ligation

material

  • bcsC and bcsD

you can see materials at protocol7

procedure

refer to protocol7

Experiment:Transformation

material

  • Competent cell(Ecos JM109)
  • bcsD in pSB1C3

you can see materials at protocol8

procedure see protocol8