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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/21
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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | ||
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===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
Revision as of 18:29, 26 October 2010
E.coli Fiber Project Notebook
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2010/10/21 Thursday (Naoto)
member naoto and watachin
Experiment:PCR
material
- templete DNA (from A.xylinum and pSB1C3)
you can see other materials at protocol3
procedure
see protocol3
Experiment:Electrophoresis
material
- PCR production(bcsA and bcsB)
you can see materials at protocol4
procedure
refer to protocol4
Experiment:DNA purification with silica gel
material
- PCR production(bcsD and pSB1C3)
you can see materials at protocol5-2
procedure
refer to protocol5-2
Experiment:Electrophoresis
material
- PCR production (bcsA,bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
see protocol4
Experiment:Digestion
material
- bcsC
- bcsD
- XbaI
- SpeI
you can see other materials at protocol6
procedure see protocol6
Experiment:DNA purification with silica gel
material
- PCR production(bcsC and bcsD)
you can see materials at protocol5-2
procedure
refer to protocol5-2
Experiment:Ligation
material
- bcsC and bcsD
you can see materials at protocol7
procedure
refer to protocol7
Experiment:Transformation
material
- Competent cell(Ecos JM109)
- bcsD in pSB1C3
you can see materials at protocol8
procedure see protocol8
