Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/20

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E.coli Fiber Project Notebook

August 2010
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2010/10/20 Wednesday (Naoto)

member naoto and watachin

Experiment:Sequence

material

  • PCR
    • bcsD in pSB1C3(No.89~96)
    • Big Dye
    • primer
    • DW
  • Ethanol precipitation
    • ethanol
    • EDTA

procedure

  1. mix materials(DNA<50ng)
  2. PCR
  3. Add EDTA and Ethanol and put at room temperature　(15min)
  4. centrifuge (8000rpm,30min)
  5. throw away supernatant
  6. add ethanol again
  7. centrifuge (8000rpm,15min)
  8. put at refrigerator
  9. throw away supernatant
  10. add Hi-Di solution
  11. transfer these sample to plate for sequence
  12. read sequence

result

Failure to read sequence


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