Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/17

From 2010.igem.org

(Difference between revisions)
Line 6: Line 6:
naoto and watachin
naoto and watachin
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===Experiment:Digestion===
+
===Experiment:Colony PCR===
'''material'''
'''material'''
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*bcsD
+
 
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*XbaI
+
*colony of E.coli
-
*SpeI
+
**bcsD:1~96
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you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
'''procedure'''
'''procedure'''
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
 
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===Experiment:DNA purification with silica gel===
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 
 +
===Experiment:Electrophoresis===
'''material'''
'''material'''
-
*PCR production(bcsD)
 
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
 
-
'''procedure'''
+
*PCR production (Colony PCR)
 +
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
'''procesure'''
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===Experiment:Ligation===
+
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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'''material'''
+
-
*bcsD
+
-
*pSB1C3
+
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
-
'''procedure'''
+
'''result'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
We can't see bands of bcsD...
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===Experiment:Transformation===
+
<br/>
-
'''material'''
+
-
*Competent cell(NovaBlue)
+
-
*bcsD in pSB1C3
+
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
-
 
+
-
'''procedure'''
+
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+

Revision as of 17:48, 26 October 2010


E.coli Fiber Project Notebook

August 2010
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2010/10/17 Sunday (Naoto)

member

naoto and watachin

Experiment:Colony PCR

material

  • colony of E.coli
    • bcsD:1~96

other materials were same as protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production (Colony PCR)

you can see other materials at protocol4

procesure

refer to protocol4

result

We can't see bands of bcsD...