Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/16

From 2010.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 1: Line 1:
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
 
+
__NOTOC__
==2010/10/16 Saturday (Naoto)==
==2010/10/16 Saturday (Naoto)==
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''material'''
+
:'''material'''
-
*PCR production (bcsA,bcsB,bcsC and bcsD)
+
:*PCR production (bcsA,bcsB,bcsC and bcsD)
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
-
'''material'''
+
:'''material'''
-
*bcsD
+
:*bcsD
-
*XbaI
+
:*XbaI
-
*SpeI
+
:*SpeI
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
===Experiment:DNA purification with silica gel===
===Experiment:DNA purification with silica gel===
-
'''material'''
+
:'''material'''
-
*PCR production(bcsD)
+
:*PCR production(bcsD)
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
===Experiment:Ligation===
===Experiment:Ligation===
-
'''material'''
+
:'''material'''
-
*bcsD
+
:*bcsD
-
*pSB1C3
+
:*pSB1C3
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
===Experiment:Transformation===
===Experiment:Transformation===
-
'''material'''
+
:'''material'''
-
*Competent cell(NovaBlue)
+
:*Competent cell(NovaBlue)
-
*bcsD in pSB1C3
+
:*bcsD in pSB1C3
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
<br/>
<br/>

Latest revision as of 17:59, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/16 Saturday (Naoto)

member
naoto and watachin

Experiment:Electrophoresis

material
  • PCR production (bcsA,bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
see protocol4

Experiment:Digestion

material
  • bcsD
  • XbaI
  • SpeI
you can see other materials at protocol6
procedure
see protocol6

Experiment:DNA purification with silica gel

material
  • PCR production(bcsD)
you can see materials at protocol5-2
procedure
refer to protocol5-2

Experiment:Ligation

material
  • bcsD
  • pSB1C3
you can see materials at protocol7
procedure
refer to protocol7

Experiment:Transformation

material
  • Competent cell(NovaBlue)
  • bcsD in pSB1C3
you can see materials at protocol8
procedure
see protocol8