Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/10

From 2010.igem.org

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__NOTOC__
__NOTOC__
==2010/10/10 Sunday (Naoto)==
==2010/10/10 Sunday (Naoto)==
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'''member'''
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:'''member'''
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watachin
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:watachin
===Preparation:Measure DNA concentration===
===Preparation:Measure DNA concentration===
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'''material'''
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:'''material'''
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you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
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'''procedure'''
+
:'''procedure'''
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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
===Experiment:Ligation===
===Experiment:Ligation===
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'''material'''
+
:'''material'''
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you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
===Experiment:Transformation===
===Experiment:Transformation===
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'''material'''
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:'''material'''
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*Competent Cell (Novablue)
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:*Competent Cell (Novablue)
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*bcsA in pSB1C3
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:*bcsA in pSB1C3
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*bcsB in pSB1C3
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:*bcsB in pSB1C3
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*bcsC in pSB1C3
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:*bcsC in pSB1C3
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*bcsD in pSB1C3
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:*bcsD in pSB1C3
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you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html>
-
'''procedure'''
+
:'''procedure'''
-
 
+
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html>
+
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html>
<br/>
<br/>

Latest revision as of 18:10, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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19 20 21 22 23 24 25
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October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/10/10 Sunday (Naoto)

member
watachin

Preparation:Measure DNA concentration

material
you can see materials at protocol5-2
procedure
refer to protocol5-2

Experiment:Ligation

material
you can see materials at protocol8
procedure
refer to protocol8

Experiment:Transformation

material
  • Competent Cell (Novablue)
  • bcsA in pSB1C3
  • bcsB in pSB1C3
  • bcsC in pSB1C3
  • bcsD in pSB1C3
you can see other materials at protocol9
procedure
refer to protocol9