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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/27
From 2010.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
:（95℃～72℃/30cycle） | :（95℃～72℃/30cycle） | ||
| - | ===Agarose Gels Electrophoresis=== | + | ===Experiment:Agarose Gels Electrophoresis=== |
:'''Member''' | :'''Member''' | ||
| Line 39: | Line 39: | ||
:Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80restriction enzyme digestion">the protocol6</a></html> | :Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80restriction enzyme digestion">the protocol6</a></html> | ||
| - | ===Agarose gel Electrophoresis=== | + | ===Experiment:Agarose gel Electrophoresis=== |
:'''Member''' | :'''Member''' | ||
| Line 50: | Line 50: | ||
:PCR Production is the above things. | :PCR Production is the above things. | ||
| - | ===Ligation=== | + | ===Experiment:Ligation=== |
:'''Member''' | :'''Member''' | ||
| Line 61: | Line 61: | ||
:*bcsC(E.coli) | :*bcsC(E.coli) | ||
| - | ===Ligation and Transformation=== | + | ===Experiment:Ligation and Transformation=== |
:'''Member''' | :'''Member''' | ||
| Line 71: | Line 71: | ||
:Same protocol on September 21st. | :Same protocol on September 21st. | ||
| - | ===PCR/bcsQ and bcsEFG(E.coli)=== | + | ===Experimet:PCR/bcsQ and bcsEFG(E.coli)=== |
:'''Member''' | :'''Member''' | ||
| Line 87: | Line 87: | ||
:（95℃～72℃/30cycle） | :（95℃～72℃/30cycle） | ||
| - | ===Agarose Gels Electrophoresis=== | + | ===Experiment:Agarose Gels Electrophoresis=== |
:'''Member''' | :'''Member''' | ||
| Line 98: | Line 98: | ||
:PCR Product of insert is the above things. | :PCR Product of insert is the above things. | ||
| - | ===PCR=== | + | ===Experiment:PCR=== |
:PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3 | :PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3 | ||
| - | ===Electrophoreses of PCR production=== | + | ===Experiment:Electrophoreses of PCR production=== |
:'''Result''' | :'''Result''' | ||
| Line 108: | Line 108: | ||
:[[Image:2010-09-27-01.jpg]] | :[[Image:2010-09-27-01.jpg]] | ||
| - | ===Digestion=== | + | ===Experiment:Digestion=== |
:bcsA → XbaI,SpeI | :bcsA → XbaI,SpeI | ||
| Line 118: | Line 118: | ||
:pSB1C3 → XbaI, SpeI | :pSB1C3 → XbaI, SpeI | ||
| - | ===Electrophoreses of PCR productions=== | + | ===Experiment:Electrophoreses of PCR productions=== |
:'''Result''' | :'''Result''' | ||
| Line 125: | Line 125: | ||
| - | ===DNA Purification=== | + | ===Experiment:DNA Purification=== |
| - | ===Ligation=== | + | ===Experiment:Ligation=== |
| - | ===DNA Purification=== | + | ===Experiment:DNA Purification=== |
| - | ===Ligation=== | + | ===Experiment:Ligation=== |
| - | ===Transfomation=== | + | ===Experiment:Transfomation=== |
| - | ===PCR=== | + | ===Experiment:PCR=== |
| - | ===Electrophoreses of PCR productions=== | + | ===Experiment:Electrophoreses of PCR productions=== |
Revision as of 15:20, 27 October 2010
E.coli Fiber Project Notebook
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2010/9/27 Monday (watachin)(bambi75)
Experiment:PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)
- Member
- watachin and bambi75
- Materials,Procedure
- Same on August 23th.
- 95℃5min
- 95℃30sec
- 55℃30sec
- 72℃4min
- 72℃5min
- 10℃∞
- （95℃～72℃/30cycle）
Experiment:Agarose Gels Electrophoresis
- Member
- Same above
- Materials and Procedure
- Same protocol the protocol4
- PCR Product of insert is the above things.
Experiment:Restriction enzyme digestion
- Member
- Same above
- Materials and Procedure
- Same protocol the protocol6
Experiment:Agarose gel Electrophoresis
- Member
- Same above
- Materials and Procedure
- Same protocol4
- PCR Production is the above things.
Experiment:Ligation
- Member
- Same above
- Materials and Procedure
- Follow the protocol(Ligation).
- bcsB(E.coli)
- bcsC(E.coli)
Experiment:Ligation and Transformation
- Member
- Same above
Materials and Procedure
- Same protocol on September 21st.
Experimet:PCR/bcsQ and bcsEFG(E.coli)
- Member
- watachin and bambi75
- Materials,Procedure
- Same on August 23th.
- 95℃5min
- 95℃30sec
- 55℃30sec
- 72℃4min
- 72℃5min
- 10℃∞
- （95℃～72℃/30cycle）
Experiment:Agarose Gels Electrophoresis
- Member
- Same above
- Materials and Procedure
- Same protocol on August 16th.
- PCR Product of insert is the above things.
Experiment:PCR
- PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
Experiment:Electrophoreses of PCR production
- Result
Experiment:Digestion
- bcsA → XbaI,SpeI
- bcsB → XbaI
- bcsC → SpeI
- pSB1C3 → XbaI, SpeI
Experiment:Electrophoreses of PCR productions
- Result
Experiment:DNA Purification
Experiment:Ligation
Experiment:DNA Purification
Experiment:Ligation
Experiment:Transfomation
Experiment:PCR
Experiment:Electrophoreses of PCR productions
Result
