Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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September 2010
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2010/09/25 Saturday(NEX)

Experiment:PCR

Member
easily
Material
  • templete DNA:pSB1C3
  • sense primers of pSB1C3
  • antisense primers of pSB1C3
Procedure
Refer to protocol3

Experiment:Ligatiom

Member
easily
Material
  • bcsA from E.coli 20μl
  • bcsB from E.coli 20μl
  • 2×ligation mix 40μl
Procedure
Follow to protocol7

Experiment:Agarose gel electrophoresis

Member
easily
Material
  • Today's PCR productions
  • Today's ligation productions
  • Agarose gel
Procedure
Refer to protocol4
Result

100925.jpg

pSB1C3 were appeared

Experiment:DNA purification with silica gel

Member
easily
Materials
  • pSB1C3
Procedure
See protocol6

Experiment:Ligatiom

Member
easily
Material
  • pSB1C3 3μl
  • bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
  • 2×ligation mix 6μl
Procedure
Follow to protocol7

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