From 2010.igem.org
E.coli Fiber Project Notebook
2010/08/26 Thursday(Bambi75)
Experiment:PCR
- Member
- NEX , Bambi75 and watachin
- Materials
- 2×PCR buffer 25×4μl
- 2mM dNTP 10×4μl
- 10mM primer(sense)bcsA,B,C and D 2.5μl each
- 10mM primer(antisense)bcsA,B,C and D 2.5μl each
- template DNA a little
- Q water 9×4μl
- KOD FX 0.5×4μl
- Procedure
- ①mix all materials for 4 tubes.
- ②elongation
- bcsA,bcsB and bcsC
- 94℃ 2min
- 98℃ 10sec☆
- 55℃ 30sec
- 68℃ 4min★
- 68℃ 7min
- 10℃ ∞
- bcsD
- 94℃ 2min
- 98℃ 10sec☆
- 55℃ 30sec
- 68℃ 1min★
- 68℃ 7min
- 10℃ ∞
- ※30cycle ☆ to ★.