Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto
Materials and Procedure
E.coli introduced bcsA,B,C,D
follow protocol3

Experiment:Plating

Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Colony PCR

Member
naoto
Materials and Procedure
Colony PCR production of bcs genes
follow protocol3

Experiment:Insert check Electrophoresis

Member
watachin
Materials and Procedure
follow protocol5
Result

2010-09-23-01.jpg 2010-09-23-02.jpg

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
  • Colony PCR production
follow protocol4
Result

2010-09-23-03.jpg

Experiment:Transformation

Member
watachin
Materials and Procedure
follow protocol9

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
follow protocol4
Result

2010-09-23-04.jpg

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5
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