Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member
NEX , bambi75 and watachin
Materials,Procedure
Same protocol on August 16th.
PCR Product of insert are bcsA and bcsC.

Experiment:PCR

Member
NEX , bambi75 and watachin
Material
  • sterilized water 213μl
  • Ex taq buffer 30μl
  • dNTP 24μl
  • Ex taq 3μl
  • bcsA sense 15μl
  • bcsA antisense 15μl
Procedure
Please see protocol3

Experiment:Restriction enzyme digestion

Member
Same above
Materials
  • PCR production
  • M buffer 30μl
  • BSA 30μl
  • Xba1 6μl
  • Spe1 6μl
Procedure
Please see protocol7

Experiment:Transformation

Member
Same above
Materials , Procedure
Please refer to protocol9

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