"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/06
From 2010.igem.org
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/10/06 Wednesday (Naoto)
Experiment:Electrophoresis of insert check PCR
- member
- naoto
- material
- insert check PCR production(PCR at 10/05)
- If you want to know other materials,see protocol4
- procedure
- see protocol4
- result
- All of bands seemed to be vector self ligation
Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase
- member
- naoto
- material
- A colony of A.xylinum
- bcsEFG(E.coli)
- T7 polymerase(BBa_K145001)
- If you want to know other materials,see protocol3
- procedure
- see protocol3
Experiment:Measure DNA density
- member
- naoto
- material
- absorption spectrometer
- bcsA,B,C,D(A.xylinum)
- bcsA,B,C,EFG(E.coli)
- pSB1C3
- TE buffer
- procedure
- mix 2µl DNA solution with 2μl TE buffer
- apply DNA solution and TE buffer mixture to absorption spectrometer
- measure DNA density
