Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14

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E.coli Fiber Project Notebook

August 2010
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2010/10/14 Thursday (Naoto)

member
naoto,maki and watachin

Preparation:Measure DNA concentration

Material
  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
  • TE buffer
Procedure
  1. mix 4μl of DNA and 4μl of TE buffer
  2. set mixture in absorption spectrometer
  3. measure DNA concentrations

Experiment:PCR

material
  • templete DNA (from A.xylinum and pSB1C3)
you can see other materials at protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
you can see materials at protocol4
procedure
refer to protocol4

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