Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/06

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E.coli Fiber Project Notebook

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October 2010
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2010/10/06 Wednesday (Naoto)

Experiment:Electrophoresis of insert check PCR

member
naoto
material
  • insert check PCR production(PCR at 10/05)
If you want to know other materials,see protocol4
procedure
see protocol4
result
All of bands seemed to be vector self ligation

Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase

member
naoto
material
  • A colony of A.xylinum
  • bcsEFG(E.coli)
  • T7 polymerase(BBa_K145001)
If you want to know other materials,see protocol3
procedure
see protocol3

Experiment:Measure DNA density

member
naoto
material
  • absorption spectrometer
  • bcsA,B,C,D(A.xylinum)
  • bcsA,B,C,EFG(E.coli)
  • pSB1C3
  • TE buffer
procedure
  • mix 2µl DNA solution with 2μl TE buffer
  • apply DNA solution and TE buffer mixture to absorption spectrometer
  • measure DNA density
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