"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29
From 2010.igem.org
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/09/29 Wednesday (Naoto)
Experiment:Insert check PCR
member
naoto and NEX
material
- bcsC,D(A.xylinum) in colonies of E.coli
If you want to know other materials,refer to protocol3
procedure
see protocol3
Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase
member
naoto and NEX
material
- A colony of A.xylinum(for templete DNA)
- bcsE~G(E.coli)
- bcsQ(E.coli)
- T7polymerase(BBa_K145001)
If you want to know other materials,refer to protocol3
procedure
see protocol3
Experiment:digestion of bcsB(E.coli)
member
naoto and easily
material
- bcsB(E.coli)
If you want to know other materials,refer to protocol6
procedure
follow to protocol6
Experiment:subculture of A.xylinum
member
naoto and easily
material
see protocol1 A.xylinum(subcultured at 9/1)
procedure
see protocol1
Experiment:Transformation
member
naoto and easily
material
- parts of bcsA,B(A.xylinum)
If you want to know other materials,refer to protocol8
procedure
see protocol8
Experiment:Electrophoresis of insert check PCR
member
naoto
material
- PCR production (bcsC,D(A.xylinum) )
If you want to know other materials,refer to protocol4
procedure
see protocol4
result
Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly
So we prepared precultures for miniprep
Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)
member
naoto
material
- PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))
If you want to know other materials,refer to protocol4
procedure
see protocol4
result
All bands don't have collect length.
