Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/27

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E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/9/27 Monday (watachin)(bambi75)

Experiment:PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)

Member
watachin and bambi75
Materials,Procedure
Same on August 23th.
  • 95℃5min
  • 95℃30sec
  • 55℃30sec
  • 72℃4min
  • 72℃5min
  • 10℃∞
(95℃~72℃/30cycle)

Experiment:Agarose Gels Electrophoresis

Member
Same above
Materials and Procedure
Same protocol the protocol4
PCR Product of insert is the above things.

Experiment:Restriction enzyme digestion

Member
Same above
Materials and Procedure
Same protocol the protocol6

Experiment:Agarose gel Electrophoresis

Member
Same above
Materials and Procedure
Same protocol4
PCR Production is the above things.

Experiment:Ligation

Member
Same above
Materials and Procedure
Follow the protocol(Ligation).
  • bcsB(E.coli)
  • bcsC(E.coli)

Experiment:Ligation and Transformation

Member
Same above

Materials and Procedure

Same protocol on September 21st.

Experimet:PCR/bcsQ and bcsEFG(E.coli)

Member
watachin and bambi75
Materials,Procedure
Same on August 23th.
  • 95℃5min
  • 95℃30sec
  • 55℃30sec
  • 72℃4min
  • 72℃5min
  • 10℃∞
(95℃~72℃/30cycle)

Experiment:Agarose Gels Electrophoresis

Member
Same above
Materials and Procedure
Same protocol on August 16th.
PCR Product of insert is the above things.

Experiment:PCR

PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3

Experiment:Electrophoreses of PCR production

Result
2010-09-27-01.jpg

Experiment:Digestion

bcsA → XbaI,SpeI
bcsB → XbaI
bcsC → SpeI
pSB1C3 → XbaI, SpeI

Experiment:Electrophoreses of PCR productions

Result

2010-09-27-02.jpg


Experiment:DNA Purification

Experiment:Ligation

Experiment:DNA Purification

Experiment:Ligation

Experiment:Transfomation

Experiment:PCR

Experiment:Electrophoreses of PCR productions

Result

2010-09-27-03.jpg