Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

E.coli Fiber Project Notebook

2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto

Materials and Procedure

Direct PCR of bcs genes

follow the protocol(Direct PCR)

Experiment:Plating

Member
watachin

Materials and Procedure

plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Insert check PCR

Member
naoto

Materials and Procedure

Insert check of bcs genes

follow the protocol(Direct PCR)

Experiment:Insert check Electrophoresis

Member
watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:DNA purification from agarose gel with QIAGEN

Member
naoto and watachin

Materials and Procedure

follow the protocol(DNA purification from agarose gel with QIAGEN)

Experiment:Insert check Electrophoresis

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:Transformation

Member
watachin

Materials and Procedure

follow the protocol(Transformation)

Experiment:Electrophoresis

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:DNA purification from agarose gel with QIAGEN

Member
naoto and watachin

Materials and Procedure