Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/01

From 2010.igem.org

Revision as of 22:01, 26 October 2010 by Naoto (Talk | contribs)


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/9/1 Wednesday(Naoto)

Experiment:Digestion of bcsA,B,C and D

Member
naoto
Material
  • bcsA,B,C,D
  • 10×Mbuffer
  • BSA
  • XbaI
  • SpeI
Procedure
  1. add "material" to PCR tubes(show below)
  2. incubation at (37℃,7h)
composition
bcsAbcsBbcsCbcsD
solution of bcs(μl) 20202020
10×Mbuffer(μl) 2 2 2 2
BSA(μl) 2 2
XbaI(μl) 0.8 0.8
SpeI(ul) 0.8 0.8

Preparation:Subculture ofA.xylinum

Member
naoto
Material
A.xylinum JCM7664
Procedure
transfer A.xylinum JCM7664 to new culture(OWW and Broth 8/25 made)

Experiment:PCR

Member
naoto
Material
  • E.coli K12 strain
  • 2×PCR buffer 25μl×2
  • 2mM dNTP 10μl×2
  • 10μM primer(sense)bcsA,B 2.5μl each
  • 10μM primer(antisense)bcsA,B 2.5μl each
  • milli-Q water 9μl×2
  • KOD FX 0.5μl×2
Procedure
follow protocol3

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/01"