Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/20
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/10/20 Wednesday (Naoto)
member naoto and watachin
Experiment:Sequence
material
- PCR
- bcsD in pSB1C3(No.89~96)
- Big Dye
- primer
- DW
- Ethanol precipitation
- ethanol
- EDTA
procedure
- mix materials(DNA<50ng)
- PCR
- Add EDTA and Ethanol and put at room temperature (15min)
- centrifuge (8000rpm,30min)
- throw away supernatant
- add ethanol again
- centrifuge (8000rpm,15min)
- put at refrigerator
- throw away supernatant
- add Hi-Di solution
- transfer these sample to plate for sequence
- read sequence
result
Failure to read sequence