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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/23
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/10/23 Saturday (Naoto)
member
naoto and watachin
Experiment:Colony PCR
material
- colony of E.coli
- bcsB:1~32
- bcsC:33~64
- bcsD:65~96
other materials were same as protocol3
procedure
see protocol3
result
From the length of bands
bcsC→No.54 and 64
bcsD→No.75
seem to be correct inserts
Preparation:making preculture
material
- Colony of No.54,64,75
- LB+Cam Broth
procedure
- Pick up a colony and inoculate into LB+Cam broth
