Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/23
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/10/23 Saturday (Naoto)
member
naoto and watachin
Experiment:Colony PCR
material
- colony of E.coli
- bcsB:1~32
- bcsC:33~64
- bcsD:65~96
other materials were same as protocol3
procedure
see html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
result
From the length of bands
bcsC→No.54 and 64
bcsD→No.75
seem to be correct inserts