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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/09/22 Wednesday (Naoto)
Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
member
naoto and NEX
material
- bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
you can see other materials at protocol3
procedure
protocol3
Experiment:Insert check PCR
member
naoto and NEX
material
- Colonies of E.coli having bcsA,B(A.xylinum)
you can see other materials at protocol3
procedure
protocol3
Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
member
naoto and NEX
material
- PCR production (bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
If you want to know other materials,refer to protocol4
procedure
see protocol4
result
Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen
Experiment:miniprep parts of bcsB,C(E.coli)
member
naoto and NEX
material
- bcsB,C(E.coli) in pSB1C3
If you want to know other materials,refer to protocol9
procedure
see protocol9
Experiment:Insert check PCR
member
naoto and NEX
material
- Insert check PCR production (bcsA,B(A.xylinum))
you can see other materials at protocol3
procedure
protocol3
result
a band of No.41(bcsB,Axylinum) appeared slightly
Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN
member
naoto and NEX
material
- bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
you can see other materials at protocol5
procedure
see protocol5
Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)
member
naoto and NEX
material
- bcsB(A.xylinum) and bcsA,B,C(E.coli)
you can see other materials at protocol6
procedure
see protocol6
Experiment:Subculture E.coli having bcsB(A.xylinum)
member
naoto and NEX
material
- LB+Cam Broth
- a colony of E.coli having bcsB(A.xylinum)
you can see other materials at protocol1
procedure
see protocol1
