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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/09/16 Thuesday (Bambi75)
Experiment:PCR
member
naoto and bambi75
materials,Procedure
Same protocol4
- 95℃3min
- 96℃1min
- 51℃1min
- 72℃3min
- 10℃∞
（96℃～72℃/30cycle）
Experiment:Agarose Gels Electro-phoresis
member
Same above
Materials,Procedure
Same protocol4 PCR Product of insert is pBS1C3
Experiment:Experiment:Make Agarose Gels Electro-phoresis
Member
Same above
Materials and Procedure
Same protocol4
Preparation:Medium making
member
Same above
materials and procedure
Same protocol1
Experiment:Ligation
member
Same above
materials and procedure follow protocol8
- bcsA,B and C(E.coli).
- bcsA,B,C and D(A.xylium)
- pSB1C3
