Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

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E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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Contents

2010/09/16 Thuesday (Bambi75)

Experiment:PCR

member

naoto and bambi75

materials,Procedure

Same protocol4

  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞

(96℃~72℃/30cycle)

Experiment:Agarose Gels Electro-phoresis

member

Same above

Materials,Procedure Same protocol on August 16th. PCR Product of insert is pBS1C3

Experiment:Experiment:Make Agarose Gels Electro-phoresis

Member

Same above

Materials and Procedure

Same protocol4

Preparation:Medium making

member

Same above

materials and procedure

Same protocol1


Experiment:Ligation

member

Same above

materials and procedure follow protocol8

  • bcsA,B and C(E.coli).
  • bcsA,B,C and D(A.xylium)
  • pSB1C3