Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

E.coli Fiber Project Notebook

2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member NEX , bambi75 and watachin

Materials,Procedure Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.

Experiment:PCR

member

NEX , bambi75 and watachin

material

• sterilized water 213μl
• Ex taq buffer 30μl
• dNTP 24μl
• Ex taq 3μl
• K12bcsA sense 15μl
• K12bcsA antisense 15μl

Procedure Please see protocol3

Experiment:Restriction enzyme digestion

member

Same above

Materials

• PCR production
• m buffer 30μl
• BSA 30μl
• Xba1 6μl
• Spe1 6μl

Procedure

Please see protocol4

Experiment:Transformation

member

Same above

Materials , Procedure