Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

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E.coli Fiber Project Notebook

August 2010
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Contents

2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member NEX , bambi75 and watachin

Materials,Procedure Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.

Experiment:PCR

member

NEX , bambi75 and watachin

material

  • sterilized water 213μl
  • Ex taq buffer 30μl
  • dNTP 24μl
  • Ex taq 3μl
  • K12bcsA sense 15μl
  • K12bcsA antisense 15μl

Procedure Please see protocol3


Experiment:Restriction enzyme digestion

member

Same above

Materials

  • PCR production
  • m buffer 30μl
  • BSA 30μl
  • Xba1 6μl
  • SPE1 6μl

Procedure

Please see protocol4


Experiment:Transformation

member

Same above

Materials , Procedure

Please refer to protocol9