Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23
From 2010.igem.org
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E.coli Fiber Project Notebook
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2010/8/23 Monday (watachin)
Experiment:Direct PCR(K12-bcsA,K12-bcsC)
Member
Bambi75 and watachin
Materials
- E.coli K12 colony
- 10µM/l forward Primer 5µl(K12-bcsA,K12-bcsC)
- 10µM/l reverse Primer 5µl(K12-bcsA,K12-bcsC)
- 10×EX taq buffer 10µl
- 2.5mM each dNTP mixture 10µl
- EX taq polymerase 1µl
- milli-Q 71µl
Procedure
follow the protocol3
Experiment:Making media(LB broth + amp)
Member
Bambi75 and watachin
Material
- LB media 200ml
- Distilled water 200ml
- LB Broth 4g
- ampicillin (50mg/ml) 200μl
Experiment:Restriction enzyme digestion
Member