Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

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E.coli Fiber Project Notebook

August 2010
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Contents

Experiment:restriction enzyme digestion

member

NEX , bambi75 and watachin

Materials

  • PCR products 50μl×3
  • m buffer 15μl
  • BSA 1 15μl
  • Xba1 3μl
  • SPE1 3μl

Equipment

  • pipet
  • pipet tip

Procedure

1)mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.

2)incubation(37℃).
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