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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/9/23 Thursday (watachin)
Experiment:PCR
Member
naoto
Materials and Procedure
Direct PCR of bcs genes
follow the protocol(Direct PCR)
Experiment:Plating
Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Insert check PCR
Member
naoto
Materials and Procedure
Insert check of bcs genes
follow the protocol(Direct PCR)
Experiment:Insert check Electrophoresis
Member
watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:Electrophoresis of bcs genes
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:DNA purification from agarose gel with QIAGEN
Member
naoto and watachin
Materials and Procedure
follow the protocol(DNA purification from agarose gel with QIAGEN)
Experiment:Insert check Electrophoresis
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:Transformation
Member
watachin
Materials and Procedure
follow the protocol(Transformation)
Experiment:Electrophoresis
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:DNA purification from agarose gel with QIAGEN
Member
naoto and watachin
Materials and Procedure
