Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/18

E.coli Fiber Project Notebook

2010/09/18 Saturday (Naoto)

Experiment:Electrophoresis

member

naoto and easily

material

• PCR production(pSB1C3)

If you want to know other materials,refer to protocol4

procedure

follow to protocol4

result

Bands didn't appear

Experiment:Insert check PCR

member

naoto and easily

material

• Parts of bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli) in colonies of E,coli

If you want to know other materials,refer to protocol3

procedure

follow to protocol3

Experiment:Direct PCR

member

naoto and easily

material

• bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3

If you want to know other materials,refer to protocol3

procedure

follow to protocol3

Experiment:Electrophoresis of bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3

member

naoto and easily

material

• PCR production(bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3)

If you want to know other materials,refer to protocol4

procedure

follow to protocol4

result

Only band of bcsA(E.coli) could be seen

Experiment:Purification bcsA(E.coli)

member

naoto and easily

material

• bcsA(E.coli) in agarose gel

If you want to know other materials,refer to protocol5

procedure

follow to protocol5

Experiment:Electrophoresis of insert check PCR

member

naoto and easily

material

• PCR production (parts of bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli) in colonies of E,coli)

If you want to know other materials,refer to protocol4

procedure

follow to protocol4

result

Bands of bcsB,C(A.xylinum) appreared

• 1~8 bcsA(A.xylinum)
• 9~12 bcsB(A.xylinum)
• 13 bcsC(A.xylinum)
• 14~15 bcsD(A.xylinum)
• 16~20 bcsA(E.coli)