Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/17

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E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/17 Sunday (Naoto)

member

naoto and watachin

Experiment:Digestion

material

  • bcsD
  • XbaI
  • SpeI

you can see other materials at protocol6

procedure see protocol6

Experiment:DNA purification with silica gel

material

  • PCR production(bcsD)

you can see materials at protocol5-2

procedure

refer to protocol5-2

Experiment:Ligation

material

  • bcsD
  • pSB1C3

you can see materials at protocol7

procedure

refer to protocol7

Experiment:Transformation

material

  • Competent cell(NovaBlue)
  • bcsD in pSB1C3

you can see materials at protocol8

procedure

see protocol8
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