Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14

From 2010.igem.org

Revision as of 17:07, 26 October 2010 by Naoto (Talk | contribs)


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/14 Thursday (Naoto)

member

naoto,maki and watachin

Preparation:Measure DNA concentration

Material

  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
  • TE buffer

Procedure

  1. mix 4μl of DNA and 4μl of TE buffer
  2. set mixture in absorption spectrometer
  3. measure DNA concentrations

Experiment:PCR

material

  • templete DNA (from A.xylinum and pSB1C3)

you can see other materials at protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • bcsA
  • bcsB
  • bcsC
  • bcsD

you can see materials at protocol4

procedure

refer to protocol4


Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/14"