Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03

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E.coli Fiber Project Notebook

August 2010
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September 2010
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Contents

2010/10/03 Sunday (Naoto)

Experiment:PCR of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum)

If you want to know other materials, see protocol3

procedure

see protocol3

Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13

member

naoto and watachin

marerial

  • bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13

If you want to know other materials, see protocol4

procedure

see protocol4

Experiment:Miniprep of BBa_K145001

member

naoto and watachin

marerial

  • Preculture of E.coli having BBa_K145001

If you want to know other materials, see protocol9

procedure

see protocol9

Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)

member

naoto and watachin

marerial

  • pSB1C3
  • bcsC,D(A.xylinum),bcsA(E.coli)

If you want to know other materials, see protocol7

procedure

see protocol7

Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)

member

naoto and watachin

marerial

  • parts of bcsC,D(A.xylinum),bcsA(E.coli)

If you want to know other materials, see protocol8

procedure

see protocol8

Experiment:Electrophoresis of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum)

If you want to know other materials, see protocol4

procedure

see protocol4

result

Band of bcsA could be seen clearly

Experiment:Purification of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum) in agarose gel

If you want to know other materials, see protocol5

procedure

see protocol5