Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09

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E.coli Fiber Project Notebook

August 2010
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September 2010
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2010/09/09 Thursday (Naoto)

Experiment:Ligation

member

naoto and NEX

material

  • bcsA,B(from A.xylinum) 10µl each
  • bcsC,D(from A.xylinum) 10µl each
  • bcsA,B(from E.coli) 10µl each
  • ligation Mix(nippon gene) 20µl each

procedure

  1. add material to PCR tube
  2. incubate (20℃　10min)

Experiment:Electrophoresis

member

naoto and NEX

material

  • ligation products(see above)

we show other material at protocol4

procedure

see protocol3
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