Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

E.coli Fiber Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/09/22 Wednesday (Naoto) 1.1 Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3 1.2 Experiment:Insert　check PCR 1.3 Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3 1.4 Experiment:miniprep parts of bcsB,C(E.coli) 1.5 Experiment:Insert　check PCR 1.6 Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN 1.7 Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli) 1.8 Experiment:Subculture E.coli having bcsB(A.xylinum)

2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

member

naoto and NEX

material

• bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

you can see other materials at protocol3

procedure

protocol3

Experiment:Insert　check PCR

member

naoto and NEX

material

• Colonies of E.coli having bcsA,B(A.xylinum)

you can see other materials at protocol3

procedure

protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

member

naoto and NEX

material

• PCR production (bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:miniprep parts of bcsB,C(E.coli)

member

naoto and NEX

material

• bcsB,C(E.coli) in pSB1C3

If you want to know other materials,refer to protocol9

procedure

see protocol9

Experiment:Insert　check PCR

member

naoto and NEX

material

• Insert check PCR production (bcsA,B(A.xylinum))

you can see other materials at protocol3

procedure

protocol3

result

a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN

member

naoto and NEX

material

• bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel

you can see other materials at protocol5

procedure

see protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

member

naoto and NEX

material

• bcsB(A.xylinum) and bcsA,B,C(E.coli)

you can see other materials at protocol6

procedure

see protocol6

Experiment:Subculture E.coli having bcsB(A.xylinum)

member

naoto and NEX

material

• LB+Cam　Broth
• a colony of E.coli having bcsB(A.xylinum)

you can see other materials at protocol1

procedure

see protocol1