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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/18
From 2010.igem.org
E.coli Fiber Project Notebook
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Contents |
2010/09/18 Saturday (Naoto)
Experiment:Electrophoresis
member
naoto and easily
material
- PCR production(pSB1C3)
If you want to know other materials,refer to protocol4
procedure
follow to protocol4
result
Bands didn't appear
Experiment:Insert check PCR
member
naoto and easily
material
- Parts of bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli) in colonies of E,coli
If you want to know other materials,refer to protocol3
procedure
follow to protocol3
Experiment:Direct PCR
member
naoto and easily
material
- bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3
If you want to know other materials,refer to protocol3
procedure
follow to protocol3
Experiment:Electrophoresis of bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3
member
naoto and easily
material
- PCR production(bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3)
If you want to know other materials,refer to protocol4
procedure
follow to protocol4
result
Only band of bcsA(E.coli) could be seen
Experiment:Purification bcsA(E.coli)
member
naoto and easily
material
- bcsA(E.coli) in agarose gel
If you want to know other materials,refer to protocol5
procedure
follow to protocol5
Experiment:Electrophoresis of insert check PCR
member
naoto and easily
material
- PCR production (parts of bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli) in colonies of E,coli)
If you want to know other materials,refer to protocol4
procedure
follow to protocol4
result
Bands of bcsB,C(A.xylinum) appreared
- 1~8 bcsA(A.xylinum)
- 9~12 bcsB(A.xylinum)
- 13 bcsC(A.xylinum)
- 14~15 bcsD(A.xylinum)
- 16~20 bcsA(E.coli)
