Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

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E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/08/13 Friday (bambi75)

Experiment:Restrict enzyme digestion

Member
NEX , bambi75 and watachin
Materials
  • bcsA,B,C,D(each):50μl
  • 10×M buffer:15μl
  • BSA:15μl
  • XbaI:3μl
  • SpeI:3μl
Procedure
  1. mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).

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