"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25
From 2010.igem.org
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/09/25 Saturday(NEX)
Experiment:PCR
- Member
- easily
- Material
- templete DNA:pSB1C3
- sense primers of pSB1C3
- antisense primers of pSB1C3
- Procedure
- Refer to protocol3
Experiment:Ligatiom
Member
easily
Material
- bcsA from E.coli 20μl
- bcsB from E.coli 20μl
- 2×ligation mix 40μl
Procedure
Follow to protocol7
Experiment:Agarose gel electrophoresis
Member
easily
Material
- Today's PCR productions
- Today's ligation productions
- Agarose gel
Procedure
Refer to protocol4
Result
pSB1C3 were appeared
Experiment:DNA purification with silica gel
Member
easily
Materials
- pSB1C3
Procedure
See protocol5-2
Experiment:Ligatiom
Member
easily
Material
- pSB1C3 3μl
- bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
- 2×ligation mix 6μl
Procedure
