Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/23

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E.coli Fiber Project Notebook

August 2010
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October 2010
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2010/10/23 Saturday (Naoto)

member

naoto and watachin

Experiment:Colony PCR

material

  • colony of E.coli
    • bcsB:1~32
    • bcsC:33~64
    • bcsD:65~96

other materials were same as protocol3

procedure

see protocol3

result

From the length of bands

bcsC→No.54 and 64

bcsD→No.75

seem to be correct inserts

Preparation:making preculture

material

  • Colony of No.54,64,75
  • LB+Cam Broth

procedure

  • Pick up a colony and inoculate into LB+Cam broth