Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

E.coli Fiber Project Notebook

2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member

naoto and bambi75

Materials,Procedure

Same protocol4

• 95℃3min
• 96℃1min
• 51℃1min
• 72℃3min
• 10℃∞

（96℃～72℃/30cycle）

Experiment:Agarose Gel Electrophoresis

Member

Same above

Materials,Procedure

Same protocol4

PCR production (pBS1C3)

Preparation:Make Agarose Gel Electrophoresis

Member

Same above

Materials and Procedure

Same protocol4

Preparation:Medium making

Member

Same above

Materials and Procedure

Same protocol1

Experiment:Ligation

Member

Same above

Materials and Procedure

follow protocol8

• bcsA,B,C and D
• pSB1C3