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Team:Bielefeld-Germany/Results
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| - | = | + | = Results = |
| - | On the next pages we are going to present you our | + | On the next pages we are going to present you our BioBrick work. |
| + | One approach of our project was to visualize a read-out by a light emitting system. So a luciferase BioBrick was build. This BioBrick is a highly sensitive reporter gene which was successfully applied in different devices with a PoPS output. The characterization of this BioBrick can be found [[Team:Bielefeld-Germany/Results/Characterization/K389004 | here]]. | ||
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| + | The second step was to establish a complete VirA/G signaling system from ''Agrobacterium tumefaciens'' in ''Escherichia coli''. For this purpose three new BioBricks were created - ''virA'', ''virG'' and a ''vir'' promoter. The VirA receptor recognizes the phenolic substance acetosyringone and transmits this information to the VirG response regulator - a bacterial transcription factor which activates ''vir'' promoters. This natural VirA/G signaling system works in ''E. coli'' and was measured and characterized by using the reporter genes [[Team:Bielefeld-Germany/Results/Characterization/K389016 | mRFP]] and [[Team:Bielefeld-Germany/Results/Characterization/K389015 | luciferase]]. | ||
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| + | In the third step ... screening device! | ||
We entered the following BioBricks to the partsregistry: | We entered the following BioBricks to the partsregistry: | ||
<groupparts>iGEM010 Bielefeld-Germany</groupparts> | <groupparts>iGEM010 Bielefeld-Germany</groupparts> | ||
Revision as of 18:53, 27 October 2010
Results
On the next pages we are going to present you our BioBrick work.
One approach of our project was to visualize a read-out by a light emitting system. So a luciferase BioBrick was build. This BioBrick is a highly sensitive reporter gene which was successfully applied in different devices with a PoPS output. The characterization of this BioBrick can be found here.
The second step was to establish a complete VirA/G signaling system from Agrobacterium tumefaciens in Escherichia coli. For this purpose three new BioBricks were created - virA, virG and a vir promoter. The VirA receptor recognizes the phenolic substance acetosyringone and transmits this information to the VirG response regulator - a bacterial transcription factor which activates vir promoters. This natural VirA/G signaling system works in E. coli and was measured and characterized by using the reporter genes mRFP and luciferase.
In the third step ... screening device!
We entered the following BioBricks to the partsregistry:
<groupparts>iGEM010 Bielefeld-Germany</groupparts>
