Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/21

From 2010.igem.org

(Difference between revisions)
 
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__NOTOC__
__NOTOC__
==2010/10/21 Thursday (Naoto)==
==2010/10/21 Thursday (Naoto)==
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
===Experiment:PCR===
===Experiment:PCR===
-
'''material'''
+
:'''material'''
-
*templete DNA (from A.xylinum and pSB1C3)
+
:*templete DNA (from A.xylinum and pSB1C3)
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''material'''
+
:'''material'''
-
*PCR production(bcsA and bcsB)
+
:*PCR production(bcsA and bcsB)
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
===Experiment:DNA purification with silica gel===
===Experiment:DNA purification with silica gel===
-
'''material'''
+
:'''material'''
-
*PCR production(bcsD and pSB1C3)
+
:*PCR production(bcsD and pSB1C3)
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''material'''
+
:'''material'''
-
*PCR production (bcsA,bcsB,bcsC and bcsD)
+
:*PCR production (bcsA,bcsB,bcsC and bcsD)
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
-
'''material'''
+
:'''material'''
-
*bcsC
+
:*bcsC
-
*bcsD
+
:*bcsD
-
*XbaI
+
:*XbaI
-
*SpeI
+
:*SpeI
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
===Experiment:DNA purification with silica gel===
===Experiment:DNA purification with silica gel===
-
'''material'''
+
:'''material'''
-
*PCR production(bcsC and bcsD)
+
:*PCR production(bcsC and bcsD)
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
===Experiment:Ligation===
===Experiment:Ligation===
-
'''material'''
+
:'''material'''
-
*bcsC and bcsD
+
:*bcsC and bcsD
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
===Experiment:Transformation===
===Experiment:Transformation===
-
'''material'''
+
:'''material'''
-
*Competent cell(Ecos JM109)
+
:*Competent cell(Ecos JM109)
-
*bcsD in pSB1C3
+
:*bcsD in pSB1C3
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
<br/>
<br/>

Latest revision as of 18:04, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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29 30 31
September 2010
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October 2010
SUNMONTUEWEDTHUFRISAT
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31


2010/10/21 Thursday (Naoto)

member
naoto and watachin

Experiment:PCR

material
  • templete DNA (from A.xylinum and pSB1C3)
you can see other materials at protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production(bcsA and bcsB)
you can see materials at protocol4
procedure
refer to protocol4

Experiment:DNA purification with silica gel

material
  • PCR production(bcsD and pSB1C3)
you can see materials at protocol5-2
procedure
refer to protocol5-2

Experiment:Electrophoresis

material
  • PCR production (bcsA,bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
see protocol4

Experiment:Digestion

material
  • bcsC
  • bcsD
  • XbaI
  • SpeI
you can see other materials at protocol6
procedure
see protocol6

Experiment:DNA purification with silica gel

material
  • PCR production(bcsC and bcsD)
you can see materials at protocol5-2
procedure
refer to protocol5-2

Experiment:Ligation

material
  • bcsC and bcsD
you can see materials at protocol7
procedure
refer to protocol7

Experiment:Transformation

material
  • Competent cell(Ecos JM109)
  • bcsD in pSB1C3
you can see materials at protocol8
procedure
see protocol8

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/21"